Elon Musk's “ultimatum” for staff to be “extremely hardcore” and work long hours or leave might have prompted hundreds of employees to leave, and offices to shut down.
The CEO had asked Twitter employees to either commit to an “extremely hardcore” culture at the company that involves “long hours at high intensity” or leave with severance.
And according to Washingtonpost, anyone who did not sign the pledge by 5 p.m. Thursday would reportedly receive three months of severance pay.
That's not all, the office buildings of Twitter will be temporarily shut down, effective immediately, the firm has informed its staff.
The message went on to say: "Please continue to comply with company policy by refraining from discussing confidential company information on social media, with the press or elsewhere."
Twitter did not immediately respond to a request for comment from the BBC.
This week Mr Musk told Twitter staff that they had to commit to working long hours and would "need to be extremely hardcore" or leave the company, according to reports.
In an email to staff, the firm's new owner said workers should agree to the pledge if they wanted to stay, the Washington Post reported.
Those who did not sign up by Thursday 17 November would be given three months' severance pay, Mr Musk said.
Earlier this month the company said that it was cutting around 50% of its workforce.
Today's announcement that Twitter had temporarily closed its offices came amid signs that large numbers of workers have now also resigned as they have not accepted Mr Musk's new terms.
Employees have been tweeting using the hashtag #LoveWhereYouWorked and a saluting emoji to show they were leaving the firm.
One former Twitter worker who wished to remain anonymous told the BBC: "I think when the dust clears today, there's probably going to be less than 2,000 people left."
They added that everyone in their team had been terminated.
"The manager of that team, his manager was terminated. And then that manager's manager was terminated. The person above that was one of the execs terminated on the first day. So there's nobody left in that chain of command."
Before Mr Musk took control of Twitter the company had around 7,500 staff. The firm was also reported to have employed thousands of contract workers, the majority of which are understood to have been laid off.
Another person said they had resigned even though they had been prepared to work long hours.
"I didn't want to work for someone who threatened us over email multiple times about only 'exceptional tweeps should work here' when I was already working 60-70 hours weekly," they said.
The world's richest person became Twitter's chief executive after buying the firm last month in a $44bn (£37bn) deal.
In response to a question about concerns that Twitter was on the brink of shutting down after the message about Twitter's offices being closed was sent Mr Musk tweeted:
"The best people are staying, so I'm not super worried".
In separate posts, he tweeted a skull and crossbones emoji and a meme showing a gravestone with the Twitter logo on it.
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